The Protective Effects of Sodium Pentaborate Tetrahydrate Against UVB-induced Apoptosis in Human Keratinocytes

Ultraviolet radiation (UV) is an environmental carcinogen causing human skin cancer. Exposure of the to UV produces apoptotic keratinocytes called sunburn cells within epidermis. Boron, essential element for plants, has several biological properties, such as anti-cancer, anti-microbial, and anti-oxidant. In present study, possible protective effects sodium pentaborate pentahydrate (SPT) against UVB-induced apoptosis in keratinocyte cells, HaCaT, were investigated. They treated with SPT at different concentrations (7.8-125 μg/mL) 24h after UVB irradiation (20, 30 60mJ/cm2). Cell viability, annexin V assay, cell cycle analysis, apoptosis-related gene levels measured using RT-PCR. Treatment (15.6-31.25μg/mL) mJ/m2 exposure significantly increased survival. Annexin analysis demonstrated a robust effect by treatment 15.6 31.25μg/mL 30mJ/cm2 irradiation. The revealed that elevated number G0/G1 phase while G2/M phase, suggesting changes partially reversed. Furthermore, 15.6μg/mL blocked activation caspase 3, 9, Bax, p53. These results indicate exerts Thus, led strong protection death HaCaT cells.